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situ probe templates for slit1a  (Addgene inc)


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    Structured Review

    Addgene inc situ probe templates for slit1a
    The trajectories of OSNs in <t>slit1a,</t> slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.
    Situ Probe Templates For Slit1a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/situ probe templates for slit1a/product/Addgene inc
    Average 88 stars, based on 2 article reviews
    situ probe templates for slit1a - by Bioz Stars, 2026-05
    88/100 stars

    Images

    1) Product Images from "The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb"

    Article Title: The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb

    Journal: bioRxiv

    doi: 10.64898/2026.03.19.709608

    The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.
    Figure Legend Snippet: The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.

    Techniques Used: Knockdown, Two Tailed Test

    Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.
    Figure Legend Snippet: Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.

    Techniques Used: In Situ Hybridization, Knockdown, Hybridization



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    The trajectories of OSNs in <t>slit1a,</t> slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.
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    The trajectories of OSNs in <t>slit1a,</t> slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.
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    Image Search Results


    The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.

    Journal: bioRxiv

    Article Title: The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb

    doi: 10.64898/2026.03.19.709608

    Figure Lengend Snippet: The trajectories of OSNs in slit1a, slit1b, slit2 , or slit3 knockdown embryos were compared to uninjected sibling controls at 72hpf. (A, B) Neither slit2 or slit3 knockdown induced errors in DZ-projecting BacOR130-1 or CZ-projecting BacOR111-7 OSNs. (C) Neither slit1a or slit1b knockdown induced errors in CZ-projecting BacOR111-7 OSNs. (D, E, F) Similar results were obtained when both slit2 and slit3 were knocked down together or when both slit1a and slit1b were knocked down together. Statistical comparisons were performed using a two-tailed Fisher’s Exact test.

    Article Snippet: In situ probe templates for slit1a (Addgene plasmid # 61279; http://n2t.net/addgene:61279 ; RRID:Addgene_61279) and slit1b (Addgene plasmid # 61280; http://n2t.net/addgene:61280 ; RRID:Addgene_61280) were a generous gift from Chi–Bin Chien & Lara Hutson ( ).

    Techniques: Knockdown, Two Tailed Test

    Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.

    Journal: bioRxiv

    Article Title: The contribution of Robos to olfactory sensory axon targeting in the olfactory bulb

    doi: 10.64898/2026.03.19.709608

    Figure Lengend Snippet: Fluorescent in situ hybridization was performed at 48 hpf in slit2 F0 knockdown embryos. Hybridization was performed in parallel at the same time as the preparations presented in . Representative 3-µm optical maximum-intensity projections from individual preparations are shown. Upregulation of slit1a and slit3 mRNA in slit2 knockdowns was observed in 3 independent experiments.

    Article Snippet: In situ probe templates for slit1a (Addgene plasmid # 61279; http://n2t.net/addgene:61279 ; RRID:Addgene_61279) and slit1b (Addgene plasmid # 61280; http://n2t.net/addgene:61280 ; RRID:Addgene_61280) were a generous gift from Chi–Bin Chien & Lara Hutson ( ).

    Techniques: In Situ Hybridization, Knockdown, Hybridization